Effect of Combined and Isometric Exercise Training on SIRT1 Level in Elderly Women with Osteopenia

Document Type : Original article

Authors

1 PhD Candidate in Exercise Physiology, Physical Education Faculty, University of Tehran, Tehran, Iran

2 Assistant Professor of Exercise Physiology, Sport Sciences Research Institute, Tehran, Iran

3 Professor of Exercise Physiology, Physical Education Faculty, University of Tehran, Tehran, Iran

4 Associated Professor of Exercise Physiology, Physical Education Faculty, University of Tehran, Tehran, Iran

Abstract

Background and Aims: SIRT1 protein has been investigated as a key factor in many biological functions, such as longevity, oxidative stress, and bone mass in interaction with physical activity. The purpose of the present study was to compare the effect of combined and isometric exercise training on plasma level of SIRT1 in elderly women with osteopenia.
Materials and Methods: For this purpose, 45 women, aged 50-60 years, with osteopenia who were eligible for the present study, were selected and randomly divided into three groups of 15, each consisting of two exercise groups (Combined group and Isometric group) and one control group. After grouping the participants' initial blood sampling, Co group was trained 45-90 min per session (stretch, strength, endurance) and Iso group was trained 20-40 min (set, frequency, contraction time), three sessions per week, for 12 weeks. Data were analyzed using SPSS, version 24, running using paired t-test, One-way ANOVA, and Tukey post hoc test with the significance level set at 0.05.   
Results: The ELISA results showed that both exercise methods had significant effects on SIRT1 compared to baseline (p=0/01). There was a significant difference between the two methods of training with control group on SIRT 1 level in the elderly women with osteopenia (p=0/001), but the difference between the two was not statistically significant (p=0/979). 
Conclusion: It seems that elevated SIRT1 level in aged people is compensatory mechanism of stress oxidative system and decreased activity in growth factor such as IGF1 in old age. It can be concluded that combined and isometric exercise could have positive effects on SIRT1 level in elderly women with osteopenia.

Keywords

Main Subjects

References

  1. JiaO Hill،Peters JC. Commentary: physical activity and weight control. Int J Epidemiol, 2013 42:1840–2.##
  2. Akbari Boukani H. Ravasi AA, Kordi MR. The effect of an endurance training period with cellular Anti-aging purpose on telomerase enzyme content in cardiac tissue and peripheral blood lymphocytes in rats. (In persian). Journal of sport physiology. 2017; 9 (3):127-140. ##
  3. Johnell O and Kanis JA. An estimate of the worldwide prevalence and disability associated with osteoporotic fractures. Osteoporos Int, 2006; 17:1726. ##
  4. Schmeisser،K.،Mansfeld،J.،Kuhlow،D.،Weimer،S.،Priebe،S.،Heiland،I.،et al. 2013. Role of sirtuins in lifespan regulation is linked to methylation of nicotinamide. Nature Chemical Biology،vol. 9،pp. 693–700. ##
  5. Kaeberlein M،McVey M،Guarente L. The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms. Genes Dev, 2011:13, 2570–2580. ##
  6. Lora Y،Hajifathalian K،Ezzati M،Woodward M،Rimm EB،Danaei G. (2014). Metabolic mediators of the effects of body-mass index،overweight،and obesity on coronary heart disease and stroke: a pooled analysis of 97 prospective cohorts with 1.8 million participants. Lancet 383:970–83. ##
  7. Guarente L. Sirtuins in aging and disease. Cold Spring Harb Symp Quant Biol. 2007;72:483-8. ##
  8. Soltani M, Rashidlamir A, Fathi M, Moghaddam M. The Effect of Eight Weeks of Water Training on Sirt1, Pgc-1α and Body Fat Percentage in Obese Men. (In persian). J Babol Univ Med Sci; 20(9); Sep 2018; PP: 55-60. ##
  9. Brendon J. Gurd،Christopher G.R. Perry،George J.F. Heigenhauser،Lawrence L. Spriet،and Arend Bonen. High-intensity interval training increases SIRT1 activity in human skeletal muscle. Appl. Physiol. Nutr. Metab. 2010; 35: 350–357. ##
  10. Zainabadi K, Liu CJ, Caldwell ALM, Guarente L. SIRT1 is a positive regulator of in vivo bone mass and a therapeutic target for osteoporosis. PLoS One. 2017 Sep 22; 12(9). ##
  11. Gibran, S K; Kenawy, N; Wong, D; Hiscott, P (2007). Changes in the retinal inner limiting membrane associated with Valsalva retinopathy. British Journal of Ophthalmology. 91 (5): 701–2. ##
  12. Ma JK, Scribbans TD, Edgett BA, Boyd JC, Simpson CA, Little JP, et al. Extremely low-volume, high-intensity interval training improves exercise capacity and increases mitochondrial protein content in human skeletal muscle. Open Journal of Molecular and Integrative Physiology. 2013; 3(04): 202. ##
  13. Zarrabeitia MT, Valero C. Association Study of Sirtuin 1 Polymorphisms with Bone Mineral Density and Body Mass Index. Archives of Medical Research. 2012; 43(5): 363-368. ##
  14. Lin C, Jiang X, Dai Z, Guo X, Weng T, Wang J, et al. Sclerostin mediates bone responseto mechanical unloading through antagonizing Wnt/beta-catenin signaling. J BoneMiner Res 2009;24:1651–61. ##
  15. Abed E, Couchourel D, Delalandre A, Duva N. Low sirtuin 1 levels in human osteoarthritis subchondral osteoblasts leadto abnormal sclerostin expression which decreasesWnt/β-catenin activity. Bone 2014; 59; Pages 28-36. ##
  16. Poole KE, van Bezooijen RL, Loveridge N, Hamersma H, Papapoulos SE, Lowik CW,et al. Sclerostin is a delayed secreted product of osteocytes that inhibits bone formation. FASEB J 2005;19:1842–4. ##
  17. Kilic U, Gok O, Erenberk U and. A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: SIRT1 Gene Variants and Longevity in Human. PLOS ONE | DOI:10.1371/journal.pone.0117954 March 18, 2015. ##
  18. Hung Lai  C, Jung Ho T, Wen Kuo W and et al. Exercise training enhanced SIRT1 longevity signaling replaces the IGF1 survival pathway to attenuate aging-induced rat heart apoptosis. Age, 2014; 36(5): 9706. ##
  19. Ferrara N, Rinaldi B, Corbi G, Conti V, Stiuso P, Boccuti et al., Exercisetraining promotes SIRT1 activity in aged rats, Rejuvenation Res. 11 (2008)139–150. ##
  20. Donniacuo M, Urbanek K, Nebbioso A et al. Cardioprotective effect of a moderate and prolonged exercise traininginvolves sirtuin pathway.Life Sciences. 2019; 222(1),140-147. ##
  21. Suwa M،Argmann C،Gerhart-Hines Z،Meziane H،Lerin C،Daussin F،et al. Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1α. Cell. 2008;127(6):1109-22. ##
  22. Gibala, M.J., Little, J.P., MacDonald, M.J. and Hawley, J.A.  Physiological adaptations to low-volume, high-intensity interval training in health and disease. The Journal of Physiology, 2011; 590, 1077-1084. ##
  23. Heywood SE, Wadley GD, McConell GD. The effect of acute exercise on skeletal muscle SIRT1. Exercise Physiology and Metabolism Laboratory. 2011; 59(6), 177-184. ##
  24. King PT. Inflammation in chronic obstructive pulmonary disease and its role in cardiovascular diseaseand lung cancer. Clinical and Translational Medicine. 2015; 4(1): 68. ##
  25. Godfrin-valent et all. Sirtuin Activity in Peripheral Blood Mononuclear Cells of Patients with Osteoporosis. Med Sci Monit Basic Res. 2014; 20: 142–145. ##
The Scientific Journal of Rehabilitation Medicine
Volume 9, Issue 3
November 2020
Pages 236-246

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History

  • Receive Date: 11 August 2019
  • Revise Date: 24 December 2019
  • Accept Date: 29 December 2019
  • First Publish Date: 22 September 2020

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