تغییرات سطوح اپلین و انسولین پلاسمایی رَت‌های چاق در اثر آزمایش استقامتی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران.

2 گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، دانشگاه سمنان، سمنان، ایران.

10.22037/jrm.2020.113886.2455

چکیده

اهداف: اپلین، آدیپوکاین تازه‌کشف‌شده‌ای است که در افراد مبتلا به چاقی و مقاومت انسولین افزایش می‌یابد. به‌روشنی معلوم نیست آزمایش استقامتی چه تأثیری بر مقادیر اپلین پلاسمایی دارد. هدف پژوهش حاضر، بررسی تغییرات میزان اپلین-13، انسولین و گلوکز رَت‌های نر چاق، در اثر دوازده هفته آزمایش‌های استقامتی بود.
مواد و روش‌ها: در مطالعه حاضر، چهارده سر رَت به‌ مدت هشت هفته تحت رژیم غذایی در دسترس پرچرب دست‌ساز قرار گرفتند و با توجه به شاخص لی، چاق محسوب شدند. پس از دوره وزن‌گیری، به دو گروه کنترل و آزمایش استقامتی تقسیم شدند. گروه آزمایش به‌ مدت دوازده هفته (هر جلسه 60 دقیقه از سرعت 15 تا 30 متر در دقیقه و پنج روز در هفته) روی تردمیل بدون شیب دویدند. از روش الایزا برای آنالیز آزمایشگاهی اپلین-13، انسولین و از روش فتومتری برای آنالیز گلوکز استفاده شد.
یافته‌ها: نتایج آزمون تی مستقل نشان داد که بین مقادیر پلاسمایی گلوکز (0/0001=P)، انسولین (0/0001=P) و اپلین (0/041=P) رَت‌های نر چاق گروه کنترل و آزمایش استقامتی تفاوت معنا‌داری وجود داشت. مقایسه میانگین متغیرها نشان داد مقادیر گلوکز و انسولین در گروه آزمایش کمتر از گروه کنترل بود و مقادیر اپلین-13 در گروه آزمایش بیشتر از گروه کنترل بود. آزمون همبستگی پیرسون بین مقادیر پلاسمایی اپلین-13 و انسولین (0/07=P)، اپلین-13 و گلوکز (0/055=P) پس از 12 هفته آزمایش استقامتی رابطه معنا‌داری را نشان نداد.
نتیجه‌گیری: با توجه به یافته‌های مطالعه حاضر، به نظر می‌رسد که دوازده هفته آزمایش استقامتی موجب کاهش مقادیر پلاسمایی انسولین و گلوکز و افزایش مقادیر پلاسمایی اپلین-13 در رَت‌های نر چاق می‌شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of 12 Weeks of Endurance Training on Plasma Levels of Apelin-13, Insulin, and Glucose in Obese Male Rats

نویسندگان [English]

  • zahra Rostami Hashjin 1
  • Abbasali Gaeini 1
  • Rouhallah Haghshenas 2
1 Faculty of Physical Education and Sport Sciences, University of Tabriz,, Tabriz, Iran
2 Department of Exercise Physiology, Faculty of Humanities, University of Semnan, Semnan, Iran.
چکیده [English]

Background and Aims: Apelin is a newly discovered adipokine whose level is high in obese people with insulin resistance. It is not clear how endurance training affects apelin plasma levels. The present study aims to investigate the plasma levels of apelin-13, insulin, and glucose in obese male rats following 12 weeks of endurance training.
Methods: Samples were 14 obese male Wistar rats who received high-fat food for eight weeks to become fat based on the Lee obesity Index. The rats were then randomly divided into control (n=7) and training (n=7) groups. The training group ran on a treadmill with zero slope for 12 weeks, 5 days per week (60-min sessions at a speed of 15-30 meters per minute). The ELISA method was used for measuring the levels of apelin-13 and insulin, while photometric glucometer was used measuring the glucose level.
Results: The results of independent t-test showed that there was a significant difference between plasma levels of glucose (P=0.0001), insulin (P=0.0001) and apelin (P=0.041) in obese male rats of control group and endurance test. The results of independent t-test showed a significant difference in plasma levels of glucose, insulin, and apelin-13 between the two groups (p < 0.05). The glucose and insulin levels in the training group were lower than in the control group , while the apelin-13 level was higher in the training group. Pearson correlation test results showed no significant relationship between levels of apelin-13 and insulin (P=0.07) as between apelin-13 and glucose (P=0.055) after 12 weeks of endurance training.
Conclusion: It seems that 12 weeks of endurance training can reduce the plasma levels of insulin and glucose and increase the plasma level of apelin-13 in obese male rats.

کلیدواژه‌ها [English]

  • Obesity
  • Endurance training
  • Apelin
  • Insulin
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